High Performance Liquid Chromatography - HPLC
Test Application
High Performance Liquid Chromatography (HPLC) is used for applications in the analytical chemistry, biochemistry and many other industries. The HPLC separates mixtures by identifying the components. The components of the HPLC system may included solvent organizer, pumps, samplers, column and detector. The pump is used to move the solvent through the column and the detector analyzes the components. Some of the general configurations of HPLC systems are shown below.
Our machines are very customizable. To have a complete HPLC system, you would need a delivery system, sampler, detector, workstation, column and solvent organizer. You also have the option to add a column oven if you like. Delivery system options include Isocratic, Binary or Quaternary pumps. The sampler includes manual and autosamplers. The detector is a UV-VIS instrument while the solvent organizer has three different models that can be used. You can choose from our pre-configured machines or customize one.
The components of the HPLC system may include solvent organizer, pumps, samplers, column and detector. The pump is used to move the solvent through the column and the detector analyzes the components. Some of the general configurations of HPLC systems are shown below.Our machines are very customizable. To have a complete HPLC system, you would need a delivery system, sampler, detector, workstation, column and solvent organizer. You also have the option to add a column oven if you like. Delivery system options include Isocratic, Binary or Quaternary pumps. The sampler includes manual and auto samplers. Detector is a UV-VIS instrument while the solvent organizer has three different models that can be used. You can choose from our preconfigured machines or customize one.
Applications
Applications
Pharmaceutical Analysis
Soybean Isoflavone is a class of secondary metabolites formed in the growth of soybean. They are extracted from the plant, similar structure with the female hormone, so soybean Isoflavone also called phytoestrogen. Soybean Isoflavone can improve skin condition and osteoporosis, relieve the symptoms of menopause syndrome.
Determination of soybean Isoflavone content in Soybean Isoflavone Soft Capsule with HPLC system with a core-shell column.
Environment Protection
Phenols pollution in the environment field means that phenols compounds pollute water. Waste water with phenols is one of the greatest danger, and most widely polluted industry waste water. In this paper, 9 phenols compounds were determined by using the HPLC system. The results show that the method is accurate and sensitive.
Food Safety
Organic acids and vitamins are very important to dry matter in fruits. They usually decide the special taste of fruits and play an important role in the food science and nutrition field. Simultaneously determining the content of 8 kinds of organic acids and Vitamins in fruit with HPLC-DII Diode Array Detector.
Agriculture
Sulfa-drugs are a class of chemical medicine for disease prevention and treatment of bacterial infections. If we take sulfa-drugs standard exceeding livestock products as food for a long time, it might lead to the damage of the hematopoietic system, agranulocytosis and so on problems. So that the application limit of sulfonamides in livestock and poultry raises up and plays an important role in the food safety field.
Chemical
UV absorbers in cosmetics can reduce or absorb the ultraviolet rays, protect the skin. Excess ultraviolet radiation will irritate skin and cause skin sensibility. The application of UV absorbers is managed and controlled strictly.
Antibiotics
In the livestock farming field, all kinds of antibiotics are used for treatment of livestock disease. Tetracycline antibiotics are widely adopt based on their antibacterial effect and cheap price. Tetracycline antibiotics mainly include Terramycin, doxycycline, tetracycline and auromycin.
Features/Advantages
Features
Typical Configuration
Isocratic System with Manual Injector - HPLCI-Isocratic-M
This isocratic system could deliver one type of mobile phase. By combing the best performance cost ratio, system stability and maintenance simplicity, this system is an ideal solution for routine QA/QC analysis and GPC/SEC application. It can also be simply upgraded into a gradient system with auto-sampler to boost your productivity.
Standard Configuration:
Manual Injector, Isocratic Pump without Degasser
- HPLC-SO1 – Solvent Organizer
- HPLC-P1 – Isocratic Pump
- HPLC-S1 – Manual Injector
- HPLC-CO – Column Oven
- HPLC-DI – UV Detector
- HPLC-COC18 – C18 column (5μm,4.6mm*250mm)
- PC+Software
Binary System with Auto-sampler - HPLCIII-Binary-A
With excellent gradient precision & optimized delay volume, this binary system plus auto-sampler is ideal for complex gradient applications or be used in combination with mass spectrometry. With the convenience of auto-sampler and various detector, the user could easily get more data
Standard Configuration:
Auto-Sampler, Binary Pump with Degasser
- HPLC-SO2 – Solvent Organizer
- HPLC-P2 – Binary Pump
- HPLC-S2 – Auto Sampler
- HPLC-CO – Column Oven
- HPLC-DI – UV Detector
- HPLC-COC18 – C18 column (5μm,4.6mm*250mm)
- PC+Software
Quaternary System with Auto-sampler - HPLCV-Quaternary-A
The quaternary system possesses the advantage of great flexibility, and it can further be automatized by combing our auto-sampler. This combination could fulfil the complex requirements from pharmacy, environmental, food safety, petrochemical engineering, agricultural & farming fields. With the control from Torontech chromatography workstation, it realized full automatic analysis, report, system flush, shut down and then raised up the efficiency.
Auto-Sampler, Quaternary Pump with Degasser
Standard Configuration:
- HPLC-P3 – Quaternary Pump
- HPLC-S2 – Auto Sampler
- HPLC-CO – Column Oven
- HPLC-DI – UV Detector
- HPLC-COC18 – C18 column (5μm,4.6mm*250mm)
- PC+Software
Technical Specifications
Technical Specifications
|
Models |
Isocratic |
Binary |
Quaternary |
|||||||||||||||||||
|
Manual |
Manual |
Auto-Sampling |
Manual |
Auto-Sampling |
||||||||||||||||||
|
Detector |
HPLC-D (choose 1 out of 4 detectors) |
|||||||||||||||||||||
|
I |
II |
III |
IV |
I |
II |
III |
IV |
I |
II |
III |
IV |
I |
II |
III |
IV |
I |
II |
III |
IV |
|||
|
Solvent Organizer |
HPLC-SO1 |
HPLC-SO2 |
|
|
||||||||||||||||||
|
Pump |
HPLC-P1 |
HPLC-P2 |
HPLC-P3 |
|||||||||||||||||||
|
Sampler |
HPLC-S1 |
HPLC-S2 |
HPLC-S1 |
HPLC-S1 |
||||||||||||||||||
|
Column Oven |
HPLC-CO (option) |
|||||||||||||||||||||
|
|
||||||||||||||||||||||
|
HPLC-DI |
UV Detector |
HPLC-P1 |
Isocratic Pump |
|||||||||||||||||||
|
HPLC-DII |
DAD – Diode Array Detector |
HPLC-P2 |
Binary Pump |
|||||||||||||||||||
|
HPLC-DIII |
ELSD - Evaporative Light Scattering Detector |
HPLC-P3 |
Quaternary Pump with integrated Degasser |
|||||||||||||||||||
|
HPLC-DIV |
RID – Refractive Index Detector |
HPLC-S1 |
Manual Injector |
|||||||||||||||||||
|
HPLC-SO1 |
Solvent Organizer (Seal wash, without degasser) |
HPLC-S2 |
Auto-sampler |
|||||||||||||||||||
|
HPLC-SO2 |
Solvent Organizer (Seal wash, 2 channels degasser) |
HPLC-CO |
Column Oven (Semi-Conductor Temperature Control) |
|||||||||||||||||||
Solvent Organizer
|
Model |
HPLC-SO1 Solvent Organizer |
HPLC-SO2 Solvent Organizer |
|
|
HPLC-SO1 |
HPLC-SO2 |
|
Online Wash |
Standard Configuration |
|
|
Online Degassing |
None |
2-channel (480 μL/channel) |
Delivery Pump
|
Model |
HPLC-P1 Isocratic pump |
HPLC-P2 Binary pump |
HPLC-P3 Quaternary pump |
|
|
HPLC-P1 |
HPLC-P2 |
HPLC-P3 |
|
Online Degassing |
None |
None |
4-channel (480 μL/channel) |
|
Flow Rate Range |
0.001 mL/min – 10.000 mL/min (increment 0.001 mL/min) |
||
|
Flow Rate Accuracy |
± 0.2% |
||
|
Flow Rate Precision |
≤ 0.1% (JJG) ≤ 0.06% RSD (ASTM) |
||
|
Max. Pressure |
9,000 psi |
||
|
Pressure Pulsation |
≤ 1% or ＜10 psi |
||
|
Gradient Accuracy |
None |
± 0.5% |
|
|
Gradient Precision |
None |
± 0.5% |
|
Auto-sampler
|
Model |
HPLC-S2 Auto-Sampler |
|
Sampling Mode |
Full loop, partial loop and μl pick-up |
|
Volume of Sample Loop |
standard: 100 μL (optional:10 μL, 20 μL, 50 μL) |
|
Max. Sampling Volume |
Full loop = loop volume Partial loop = 1/2 loop volume μl pick-up = (loop volume- 3 × needle volume)/2 |
|
Sample Volume |
2*48 1.5 mL vials (standard) (Optional: 96-hole well plate, 384-hole well plate) |
|
Cross Contamination |
＜0.005% |
|
Linearity |
0.9999 |
|
Pressure Limit |
6,000 psi (15,000psi sampling valve is optional) |
Column Oven
|
Model |
HPLCHPLClumn Oven (Semi-Conductor Temperature Control) |
|
Temperature Range |
Room Temp +5℃ - 70℃ |
|
Temperature Accuracy |
± 1.0 ℃ |
|
Temperature Precision |
± 0.1 ℃ |
ELSD
|
Model |
HPLC-DIII ELSD - Evaporative Light Scattering Detector |
|
Light Source |
LED 470 nm |
|
Evaporation Temperature |
RT - 100 ℃ (increment 1℃) |
|
Flow Rate |
0.1 mL/min – 2.5 mL/min (standard) |
|
Air Consumption |
<3.0 L/min |
|
Air Required |
>5 L/min nitrogen gas or air, 2 - 4.5 Bar |
UV-VIS Detector
|
Model |
HPLC-DI UV-VIS Detector |
|
Wavelength |
190-800 nm |
|
Light Source |
Deuterium lamp, Tungsten lamp |
|
Spectral Width |
8 nm |
|
Wavelength Accuracy |
± 1 nm |
|
Wavelength Precision |
0.1 nm |
|
Noise |
± 0.5×10-5 AU (JJG) ± 0.35×10-5 AU (ASTM) |
|
Drift |
1×10-4 AU/Hr |
|
Linear Range |
> 5×104 (JJG) > 2.5AU (ASTM) |
|
Max. Sampling Rate |
100 Hz |
|
Flowcell Pressure |
1200 PSI |
|
Flowcell Light Path |
10 mm |
|
Flowcell Volume |
12 μL |
|
Detection Limit |
4×10-9 g/mL (naphthalene) |
Diode Array Detector
|
Model |
HPLC-DII Diode Array Detector |
|
Diode Array |
1024 pixels |
|
Wavelength |
190 ~ 640 nm |
|
Light Source |
Deuterium lamp |
|
Spectral Resolution |
0.6 nm/pixel |
|
Spectral Width |
4.8 nm |
|
Wavelength Accuracy |
± 1 nm |
|
Wavelength Precision |
0.1 nm |
|
Noise |
± 1.0×10-5 AU (JJG) ± 0.6×10-5 AU (ASTM) |
|
Drift |
0.5×10-3 AU/Hr |
|
Linear Range |
> 2.0 AU (ASTM) |
|
Max. Sampling Rate |
12 channels,100 Hz; Full spectrum,100 Hz |
|
Flow cell Pressure |
1200 PSI |
|
Flow cell Light Path |
10 mm |
|
Flow cell Volume |
12 μL |
|
Detection Limit |
2×10-8 g/mL (naphthalene) |
|
Wavelength Calibration |
Mercury peaks and built-in holmium oxide filter |
Refractive Index Detector
|
Model |
HPLC-DIV Refractive Index Detector |
|
Refractive Index range |
1.00 ~ 1.75 |
|
Measuring range |
0.25 ~ 512 μ RIU |
|
Linearity range |
≥600 μ RIU |
|
Response time |
0.1, 0.25, 0.5, 1, 1.5, 2, 3, 6 sec |
|
Auto zero |
Full auto zero |
|
Cell volume |
8 μL |
|
Flow rate |
(Typical) 0.2 ~3.0 mL/min (Max.) 10 mL/min (solvent: pure water) |
|
Max. pressure |
50 kpa |
|
Temp. control |
OFF, 30 ~55℃ (1℃ step), 77℃ Temp. fuse |
|
Noise |
≤2.5 nRIU (Pure water, response: 1.5 sec) |